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Related Experiment Videos

Self-compatible B mutants in coprinus with altered pheromone-receptor specificities.

N S Olesnicky1, A J Brown, Y Honda

  • 1Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.

Genetics
|November 7, 2000
PubMed
Summary

Mutations in mushroom mating genes can alter pheromone and receptor interactions. A single amino acid change in either can disrupt specificity, leading to self-compatibility and deregulated development.

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Area of Science:

  • Mycology
  • Molecular Biology
  • Genetics

Background:

  • Mating in *Coprinus cinereus* relies on compatible pheromones and G-protein-coupled receptors encoded by the B mating-type locus.
  • Mutations in B genes can cause constitutive activation of development, bypassing the need for mating.

Purpose of the Study:

  • To characterize a mutation in the B6 locus affecting pheromone-receptor recognition.
  • To investigate how single amino acid substitutions impact mating compatibility and specificity.

Main Methods:

  • Heterologous yeast assay using synthetic pheromones.
  • Analysis of a specific B6 locus mutation (R96H) in the receptor.
  • Parallel experiments in *Coprinus* with a F67W pheromone substitution.

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Main Results:

  • The R96H receptor mutation does not cause constitutive activity but allows response to an incompatible pheromone from the same locus.
  • A F67W substitution in the pheromone enables activation of the wild-type receptor.
  • Single amino acid changes in either pheromone or receptor can deregulate recognition specificity.

Conclusions:

  • Specific amino acid substitutions in pheromones or receptors can confer self-compatibility by altering ligand-receptor recognition.
  • Receptors and pheromones within a single B locus belong to distinct subfamilies, preventing normal activation between them.