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Related Experiment Videos

Developmental changes of CD34 expression by murine hematopoietic stem cells.

T Ito1, F Tajima, M Ogawa

  • 1Department of Medicine, Medical University of South Carolina Charleston, SC, USA.

Experimental Hematology
|November 7, 2000
PubMed
Summary

Hematopoietic stem cells (HSCs) in mice transition from CD34-positive to CD34-negative during development. This shift occurs between 7 and 10 weeks, with about 20% of adult HSCs remaining CD34-positive.

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Area of Science:

  • Developmental Biology
  • Hematopoiesis
  • Stem Cell Biology

Background:

  • Previous reports indicated fetal murine hematopoietic stem cells (HSCs) are CD34-positive, while adult HSCs are CD34-negative.
  • This suggests a developmental change in CD34 expression during HSC maturation.
  • Understanding this transition is crucial for stem cell research and clinical applications.

Purpose of the Study:

  • To systematically analyze the developmental changes in CD34 expression by murine HSCs.
  • To determine the age at which CD34 expression shifts from positive to negative.
  • To clarify the CD34 status of adult HSCs and its implications.

Main Methods:

  • Analysis of long-term engrafting cells in bone marrow and blood from mice of various ages (perinatal, juvenile, adult).

Related Experiment Videos

  • Isolation of CD34-positive and CD34-negative mononuclear cell populations without prior enrichment.
  • Assessment of long-term reconstituting abilities via transplantation into lethally irradiated Ly-5 congenic mice.

    • Main Results:

    • All HSCs from perinatal to 5-week-old mice were CD34-positive.
    • CD34-negative HSCs emerged at 7 weeks, becoming the majority by 10-20 weeks.
    • Approximately 20% of adult murine HSCs retained CD34 expression.

    Conclusions:

    • The majority of murine HSCs switch from CD34-positive to CD34-negative between 7 and 10 weeks of age.
    • A subset of adult HSCs (around 20%) remains CD34-positive, contributing to ongoing debate.
    • Findings suggest human umbilical cord blood and young children's bone marrow HSCs are predominantly CD34-positive.