Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eukaryotic DNA replication: from pre-replication complex to initiation complex.

H Takisawa1, S Mimura, Y Kubota

  • 1Department of Biology, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, 560-0043, Osaka, Japan. takisawa@bio.sci.osaka-u.ac.jp

Current Opinion in Cell Biology
|November 7, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Study on the Interrelationship between Information Value and X-Ray Dose in the Dental Radiogram.

Dento maxillo facial radiology·2015
Same author

Feasibility of endoscopic mucosal resection as salvage treatment for patients with local failure after definitive chemoradiotherapy for stage IB, II, and III esophageal squamous cell cancer.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2013
Same author

Cooperative Clinical Trial of Photodynamic Therapy for Early Gastric Cancer With Photofrin Injection and YAG-OPO Laser.

Diagnostic and therapeutic endoscopy·2008
Same author

A Clinical Study of Photodynamic Therapy for Superficial Esophageal Carcinoma by YAG-OPO Laser.

Diagnostic and therapeutic endoscopy·2008
Same author

Progress of photodynamic therapy in gastric cancer.

Diagnostic and therapeutic endoscopy·2008
Same author

A new technique for improving visualization of mucosal lesions during endoscopic photodynamic therapy.

Diagnostic and therapeutic endoscopy·2008
Same journal

Mechanosensing in immune cells: Implications for migration and beyond.

Current opinion in cell biology·2026
Same journal

Emerging role of organelles in cell migration.

Current opinion in cell biology·2026
Same journal

Nuclear adaptation in cell migration.

Current opinion in cell biology·2026
Same journal

Patterns in motion: Choreographing dynamic cell behaviours during tissue repair.

Current opinion in cell biology·2026
Same journal

Quo vadis reconstituted cell surfaces? Purpose and future perspectives for minimal systems of the cell plasma membrane.

Current opinion in cell biology·2026
Same journal

Nuclear determinants of mRNA and protein isoforms.

Current opinion in cell biology·2026
See all related articles

Eukaryotic DNA replication initiation involves the minichromosome maintenance (MCM) complex and Cdc45. S phase kinases orchestrate their assembly at origins, enabling DNA unwinding and replication protein recruitment for cell division.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic DNA replication is essential for cell division and requires precise initiation control.
  • The minichromosome maintenance (MCM) complex and Cdc45 are key players in replication initiation.
  • S phase promoting kinases are crucial regulators conserved across species.

Purpose of the Study:

  • To elucidate the common mechanism controlling eukaryotic DNA replication initiation.
  • To highlight the roles of MCM, Cdc45, and S phase kinases in this process.

Main Methods:

  • The study focuses on the molecular interactions and regulatory roles of key proteins.
  • Comparative analysis of conserved mechanisms in yeast and humans.

Main Results:

Related Experiment Videos

  • MCM and Cdc45 form a central initiation complex.
  • S phase kinases promote the association of Cdc45 with MCM at replication origins.
  • Formation of the MCM-Cdc45 complex leads to DNA unwinding and recruitment of replication machinery, including DNA polymerases.

Conclusions:

  • A conserved mechanism for eukaryotic DNA replication initiation has been identified.
  • The intricate machinery controlling initiation involves multiple interacting factors, underscoring its complexity and evolutionary significance.