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Strain-dependent antibody response induced by DNA immunization
K Ito1, Y Takeuchi, K Ito
1Kato Cytoprotein Network Project, ERATO, Japan Science and Technology (JST), c/o Sagami Chemical Research Center, 4-4-1 Nishi-ohnuma, 229-0012, Sagamihara, Japan. koichi@med.kitasato-u.ac.jp
Immunology Letters
|November 7, 2000
Summary
DNA immunization elicits antibody responses, but the effectiveness varies by mouse strain and the specific gene used. The major histocompatibility complex (H-2) haplotype significantly influences the antibody production, impacting DNA vaccine efficacy.
Area of Science:
- Immunology
- Genetics
- Molecular Biology
Background:
- DNA-based immunization is a novel method for inducing antibody responses.
- The mouse major histocompatibility complex (H-2) is known to influence immune responses.
- Different strains of mice possess diverse H-2 haplotypes, potentially affecting DNA vaccine outcomes.
Purpose of the Study:
- To investigate the influence of H-2 haplotypes on antibody production following DNA-based immunization.
- To compare the antibody responses induced by DNA immunization versus peptide immunization.
- To assess the correlation between gene expression at the inoculation site and antibody production.
Main Methods:
- Comparison of antibody (Ab) response in various inbred, H-2 congenic, and outbred mouse strains.
- Introduction of plasmid DNA vectors encoding green fluorescent protein (GFP) or beta-galactosidase (beta-gal) into muscle tissue.
- Analysis of Ab production using enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis.
- Detection of gene transcripts via reverse transcription-polymerase chain reaction (RT-PCR).
Main Results:
- Beta-galactosidase (beta-gal) DNA immunization induced strong early antibody responses across all tested mouse strains.
- Antibody response to beta-gal DNA immunization was H-2 haplotype-dependent, unlike beta-gal peptide immunization.
- Antibody production following green fluorescent protein (GFP) DNA immunization was observed in outbred strains but not all inbred strains.
- Despite varying antibody responses, GFP and beta-gal transcripts were detected at the DNA inoculation site in all strains.
Conclusions:
- The H-2 haplotype of the host strain is a critical determinant of antibody response efficacy for DNA immunization.
- Differences in antibody production, whether induced by DNA or peptide immunization, are significantly influenced by H-2 haplotypes.
- Gene expression at the DNA inoculation site does not solely predict the antibody response.
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