Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Apparent coactivation due to interference of expression constructs with nuclear receptor expression.

K Hofman1, J V Swinnen, F Claessens

  • 1Laboratory for Experimental Medicine and Endocrinology, Faculty of Medicine, Catholic University of Leuven, B-3000, Leuven, Belgium.

Molecular and Cellular Endocrinology
|November 7, 2000
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A deep phenotyping study in mouse and iPSC models to understand the role of oligodendroglia in optic neuropathy in Wolfram syndrome.

Acta neuropathologica communications·2024
Same author

Vasodilator reactive oxygen species ameliorate perturbed myocardial oxygen delivery in exercising swine with multiple comorbidities.

Basic research in cardiology·2024
Same author

Effect on hospital incentive payments and quality performance of a hospital pay for performance (P4P) programme in Belgium.

Journal of healthcare quality research·2024
Same author

The FlaQuM-Quickscan: A starting point to include primary care professionals' perspectives in the evaluation of hospital quality priorities.

Journal of healthcare quality research·2024
Same author

Androgen therapy does not prevent bone loss and arterial calcifications in male rats with chronic kidney disease.

The Journal of endocrinology·2023
Same author

New developments with the Health Promotion Levy in South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2022

Transient cotransfection experiments may yield misleading results regarding nuclear receptor coactivation. Researchers should measure receptor concentration to avoid misinterpreting expression changes as coactivation effects.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Nuclear receptors play crucial roles in gene regulation.
  • Coactivation is a key mechanism modulating nuclear receptor activity.
  • NuRIP183 is a novel nuclear receptor interacting protein.

Purpose of the Study:

  • To investigate the coactivation properties of NuRIP183.
  • To determine if NuRIP183 enhances nuclear receptor activity.
  • To validate cotransfection assays for studying coactivation.

Main Methods:

  • Transient cotransfection in COS-7, CV-1, and MCF-7 cells.
  • Yeast two-hybrid screening for protein interaction.
  • Reporter gene assays to measure nuclear receptor activity.

Related Experiment Videos

  • Analysis of receptor expression levels.

    • Main Results:

    • NuRIP183 expression constructs initially appeared to enhance nuclear receptor activity.
    • However, cotransfection altered endogenous nuclear receptor expression levels.
    • Observed effects were attributed to expression interference, not coactivation.
    • Interference was inversely related to expression construct insert length.

    Conclusions:

    • Standard cotransfection assays may inaccurately reflect nuclear receptor coactivation.
    • Changes in receptor concentration due to expression interference can be misinterpreted.
    • Recommendations include measuring receptor levels and optimizing transfection conditions.
    • Caution is advised for interpreting cotransfection data in similar studies.