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CD44 expression in the developing and growing rat intervertebral disc.

J W Stevens1, G L Kurriger, A S Carter

  • 1Department of Orthopaedic Surgery, The University of Iowa, Iowa City, Iowa 52242-1181, USA. jeff-stevens@uiowa.edu

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|November 7, 2000
PubMed
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CD44, a cell surface glycoprotein, is exclusively found on notochordal cells during rat intervertebral disc development. This study identifies CD44 standard (CD44s) expression in nucleus pulposus cells, linking it to extracellular matrix formation.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biochemistry

Background:

  • CD44 is involved in extracellular matrix formation during embryonic development.
  • The nucleus pulposus, a key component of the intervertebral disc, has not been thoroughly analyzed for CD44 expression.
  • Understanding CD44's role in disc development is crucial for regenerative medicine and understanding disc degeneration.

Purpose of the Study:

  • To investigate the expression and characteristics of CD44 in the developing and aging rat intervertebral disc, specifically within the nucleus pulposus.
  • To determine the cellular localization and molecular form of CD44 in this tissue.

Main Methods:

  • Immunohistochemistry to detect CD44 expression in developing and aging rat intervertebral discs.
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify the CD44 standard (CD44s) open reading frame.

Related Experiment Videos

  • Western blot analysis to characterize the molecular weight and modifications of CD44.
  • Co-localization studies with hyaluronan and proteoglycans.

    • Main Results:

    • CD44 was exclusively identified on notochordal cells from initial intervertebral disc formation (embryonic day 15) through their disappearance (12-24 months).
    • No CD44 expression was observed before disc formation or in other cell types within the annulus fibrosus or nucleus pulposus.
    • RT-PCR confirmed the presence of CD44s, and Western blot revealed an 85 kDa CD44 species lacking chondroitin sulfate.
    • Cell surface CD44 co-localized with hyaluronan and proteoglycans during early disc formation.

    Conclusions:

    • CD44 is a specific marker for notochordal cells throughout the development and aging of the rat intervertebral disc.
    • The identified CD44s form in notochordal cells is associated with the early stages of nucleus pulposus extracellular matrix formation.
    • These findings provide insights into the cellular and molecular mechanisms governing intervertebral disc development and maintenance.