Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved performance of pyrosequencing using single-stranded DNA-binding protein.

M Ronaghi1

  • 1Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, California 94304, USA. mostafa@stanford.edu

Analytical Biochemistry
|November 9, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ROPS performance during field upset and static testing.

Journal of agricultural safety and health·2010
Same author

Dynamic performance of the mechanism of an automatically deployable ROPS.

Journal of agricultural safety and health·2002
Same author

Static load test performance of a telescoping structure for an automatically deployable ROPS.

Journal of agricultural safety and health·2002
Same author

Preventing tractor rollover fatalities: performance of the NIOSH autoROPS.

Injury prevention : journal of the International Society for Child and Adolescent Injury Prevention·2001
Same author

Performance of an automatically deployable ROPS on ASAE tests.

Journal of agricultural safety and health·2001
Same author

Method enabling fast partial sequencing of cDNA clones.

Analytical biochemistry·2001
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

Adding single-stranded DNA-binding protein (SSB) to Pyrosequencing reactions significantly improved DNA sequencing performance. This enhancement led to longer reads, increased accuracy, and greater enzyme efficiency for DNA sequencing platforms.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • High-throughput and cost-effective DNA sequencing platforms are essential for modern biological research.
  • Pyrosequencing is a key DNA sequencing technology that relies on enzyme cooperation to detect DNA synthesis.

Purpose of the Study:

  • To investigate the impact of single-stranded DNA-binding protein (SSB) on Pyrosequencing performance.
  • To determine if SSB can enhance read length and accuracy in DNA sequencing.

Main Methods:

  • Pyrosequencing reactions were performed with the addition of single-stranded DNA-binding protein (SSB) to the primed DNA template.
  • The effects of SSB on enzyme efficiency, mispriming, signal intensity, and read accuracy were evaluated.

Main Results:

Related Experiment Videos

  • The inclusion of SSB in Pyrosequencing reactions resulted in DNA read lengths exceeding 30 nucleotides.
  • SSB improved enzyme efficiency, reduced nonspecific signals (mispriming), and increased signal intensity.
  • Accuracy in reading homopolymeric regions and overall read length were significantly enhanced.

Conclusions:

  • Single-stranded DNA-binding protein (SSB) is a valuable additive for improving Pyrosequencing efficiency and read length.
  • The use of SSB offers a promising strategy for developing more robust and accurate DNA sequencing platforms.