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Real-time PCR assay with fluorescent hybridization probes for rapid genotyping of the CD14 promotor polymorphism

M Heesen, D Kunz, R Rossaint

    Clinical Chemistry
    |November 9, 2000
    PubMed
    Abstract

    No abstract available in PubMed .

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    Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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