Leukocyte responses in two breeds of layer chicken that differ in susceptibility to induced amyloid arthropathy

Amyloid arthropathy in chicken can be induced by intravenous inoculation of an arthropathic and amyloidogenic Enterococcus faecalis in susceptible breeds. The commercial brown layer hybrids (BL) are more susceptible to the disease compared to their white counterparts (WL). The precursor of amyloid-A protein, which is serum amyloid-A (SAA), is identical in WL and BL. To investigate the factors involved in the breed-restricted susceptibility to amyloid arthropathy, we studied the type of leukocyte response and inflammatory reactions in E. faecalis-induced disease. In the BL, a significant dose dependent peripheral leukocytosis mainly by heterophils, and plasma cell infiltration in arthritic joints was found. In contrast, secondary lymphoid nodular aggregates in the synovial membrane were prominent in the WL. The aggregates consisted mainly of CD8+ T cells. The high number of circulating leukocyte and prolific plasma cell responses in the BL predict extensive humoral and acute phase reactions. This is in agreement with literature data on suppressed T-cell function in casein-induced amyloid-susceptible mice strains. The difference in leukocyte response and type of inflammation between WL and BL, when arthropathic and amyloidogenic bacteria induce infection, in conjunction with susceptibility to amyloid arthropathy, is discussed in view of the murine T-helper responses.