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Related Experiment Videos

The nylon column dye test: a possible screening test of phagocyte function.

A W Segal, T J Peters

    Clinical Science and Molecular Medicine
    |December 1, 1975
    PubMed
    Summary

    A new quantitative test using Nitroblue Tetrazolium effectively measures phagocyte function by detecting dye reduction. This simple assay shows promise as a screening tool for chronic granulomatous disease.

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    Area of Science:

    • Immunology
    • Clinical Chemistry

    Background:

    • Phagocyte dysfunction is central to certain immune deficiencies.
    • Accurate assessment of phagocyte function is crucial for diagnosing and managing these conditions.

    Purpose of the Study:

    • To develop a simple, quantitative assay for evaluating phagocyte activity.
    • To assess the utility of this assay as a screening method for phagocyte disorders.

    Main Methods:

    • A novel test involves adding Nitroblue Tetrazolium to whole blood.
    • Leukocytes are isolated using a nylon wool column, and dye reduction is quantified spectrophotometrically.

    Main Results:

    • Formazan formation, indicating dye reduction, correlated directly with phagocyte numbers.
    • Abnormal results were observed in two patients with chronic granulomatous disease.

    Conclusions:

    • The developed Nitroblue Tetrazolium assay is a simple and quantitative method for assessing phagocyte function.
    • This test demonstrates potential as a valuable screening tool for chronic granulomatous disease.

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