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Involvement of the beta2-integrin CD18 in apoptosis signal transduction in human neutrophils
J Larsson1, L Serrander, O Stendahl
1Division of Pathology II, Faculty of Health Sciences, Linköping University, Sweden. jenny.larsson@inr.liu.se
Objective And Design:
To examine the hypothesis that an accelerated rate of neutrophil apoptosis occurs following beta2-integrin activation, and further investigate the signal transduction pathways involved.
Material:
Human polymorphonuclear neutrophils.
Treatment:
Neutrophils were challenged with pansorbins coated with antibodies towards the beta2-integrin subunit CD18 in a proportion of 1:100 with or without the inhibitors diphenylene iodonium (10 M), cytochalasin B (5 microg/ml), genistein (10 nM), herbimycin A (10 M) and Z-VAD-FMK (10 microM).
Methods:
Measurement of phosphatidylserine exposure and DNA fragmentation in flow cytometry and assessment of H2O2-production through spectrofluorometry. The results were analysed using Mann Whitney U test and Kruskal Wallis.
Results:
Pansorbins coated with antibodies to CD18 induce apoptosis in neutrophils (p<0.01), and activate the production of reactive oxygen species (p<0.01). Pre-treatment with the inhibitors have no effect on anti-CD 18 induced apoptosis.
Conclusion:
Anti-CD18 pansorbins induce apoptosis in neutrophils through an alternative pathway not involving reactive oxygen species and independent of tyrosine phosphorylation, cytoskeletal reorganisation and caspases.
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