An efficient solid-phase strategy for the construction of chemokines

Synthesis of chemokines via stepwise SPPS approaches has been shown to be a challenge. Herein, a complete study of different coupling methods, solvents and temperature combined with a continuous-flow synthesizer equipped with feedback monitoring was carried out. The results from this study indicate that this family of molecules can be prepared using an Fmoc/Bu(t) chemical approach and provide a general method to apply for the elongation of other difficult sequences.