Related Experiment Video

Updated: Aug 26, 2026

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
09:40

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Published on: June 20, 2018

Polymorphonuclear leukocyte rigidity is defective in patients with chronic renal failure

A T Skoutelis1, V E Kaleridis, D S Goumenos

  • 1Department of Medicine, Patras University Medical School, Patras University, Patras, Greece.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|November 10, 2000
PubMed
Abstract

Insights

Polymorphonuclear leukocyte (PMN) rigidity increases in end-stage chronic renal failure (CRF) patients and further elevates during haemodialysis (HD). This study investigated PMN rigidity and inflammatory markers in CRF and HD patients.

Area of Science:

  • Nephrology
  • Immunology
  • Cell Biology

Background:

  • Chronic renal failure (CRF) impacts immune cell function.
  • Haemodialysis (HD) may further alter polymorphonuclear leukocyte (PMN) behavior.

Purpose of the Study:

  • To investigate polymorphonuclear leukocyte (PMN) rigidity in non-dialysed CRF and haemodialysis (HD) patients.
  • To assess the impact of HD on PMN rigidity and inflammatory markers.

Main Methods:

  • Assessed PMN rigidity, TNF-alpha, and IL-1beta levels in CRF and HD patients.
  • Utilized micropipette method for PMN deformability and ELISA for cytokine analysis.
  • Compared results between early-stage CRF, late-stage non-HD, HD patients, and healthy controls.

Main Results:

  • PMN rigidity was significantly increased in end-stage CRF patients, independent of HD.
  • Haemodialysis significantly increased PMN rigidity and inflammatory cytokine levels (TNF-alpha, IL-1beta) within 60 minutes.
  • The type of HD membrane did not influence PMN rigidity, activation, or cytokine production.

Conclusions:

  • PMN rigidity is impaired in end-stage CRF and exacerbated by haemodialysis.
  • Increased PMN rigidity during HD may be linked to PMN activation and elevated inflammatory cytokines.
  • Findings suggest a direct PMN impairment or inflammatory response contributes to altered cell rigidity in CRF and HD.

More Related Videos

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes11:47

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

Related Concept Videos

Disorders of Leukocytes01:27

Disorders of Leukocytes

Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...
Nephrons01:10

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma happens...
Renal Corpuscle01:20

Renal Corpuscle

The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...
Factors Affecting Renal Clearance: Renal Impairment01:17

Factors Affecting Renal Clearance: Renal Impairment

Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
Alterations in Muscle Tone lll01:11

Alterations in Muscle Tone lll

Rigidity and myotonia are distinct abnormalities of muscle tone that affect resistance and relaxation during movement. Although both involve altered muscle contraction, they arise from different neurological and muscular mechanisms.CharacteristicsRigidity is characterized by uniform resistance to passive movement across the entire range, independent of speed, affecting flexors and extensors equally. It may appear as lead-pipe rigidity (smooth, constant resistance) or cogwheel rigidity...