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Clinical isolation of Yersinia enterocolitica: cold temperature enrichment
Journal of Clinical Microbiology
|December 1, 1975
Abstract:
A cold-temperature enrichment procedure was used to isolate Yersinia enterocolitica serotype 6 from a clinical stool specimen. The use of conventional laboratory media and enrichment procedures failed to isolate this organism.
Insights
A novel cold-temperature enrichment method successfully isolated Yersinia enterocolitica serotype 6 from a clinical stool sample. Conventional methods were unsuccessful in detecting this specific bacterial pathogen.
Area of Science:
- Microbiology
- Clinical Diagnostics
- Bacteriology
Background:
- Yersinia enterocolitica is a significant human pathogen.
- Serotype 6 is a clinically relevant serotype of Yersinia enterocolitica.
- Standard diagnostic methods may not always detect all Yersinia enterocolitica serotypes.
Purpose of the Study:
- To describe a successful cold-temperature enrichment technique for isolating Yersinia enterocolitica serotype 6.
- To highlight the limitations of conventional methods for detecting specific Yersinia enterocolitica serotypes.
Main Methods:
- A clinical stool specimen was processed.
- A cold-temperature enrichment procedure was employed.
- Conventional laboratory media and enrichment procedures were utilized for comparison.
Main Results:
- Yersinia enterocolitica serotype 6 was successfully isolated using the cold-temperature enrichment method.
- Conventional laboratory media and enrichment procedures failed to isolate Yersinia enterocolitica serotype 6 from the same specimen.
Conclusions:
- Cold-temperature enrichment is a valuable technique for isolating Yersinia enterocolitica serotype 6.
- This method offers improved sensitivity compared to conventional approaches for specific serotypes.