Related Experiment Videos
Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei
B Martínez1, J Sillanpää, E Smit
1Department of Applied Microbiology and Gene Technology, TNO Voeding, 3700 AJ Zeist, The Netherlands.
Journal of Bacteriology
|November 14, 2000
Abstract:
The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393(T). The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens.