Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neuroanatomical tracing at high resolution.

T Van Haeften1, F G Wouterlood

  • 1Department of Anatomy, Faculty of Medicine, Graduate School Neurosciences Amsterdam, Institute for Neurosciences Vrije Universiteit, Amsterdam, The Netherlands. tvanhaeften@med.vu.nl

Journal of Neuroscience Methods
|November 14, 2000
PubMed
Summary

Investigating fiber connectivity in the central nervous system requires electron microscopy for synaptic-level detail. This study reviews methods to visualize individual tracers and dual markers for precise neural circuit mapping.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Terminal degeneration in the mediodorsal cerebral cortex of the lizard Agama agama: Light and electron microscopy.

Journal of morphology·2018
Same author

Kisspeptin-10 and the G protein-coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells.

Reproduction in domestic animals = Zuchthygiene·2016
Same author

Identification of genes targeted by FSH and oocytes in porcine granulosa cells.

Theriogenology·2010
Same author

A method for immediate comparative assessment of microinjected mammalian oocytes.

Theriogenology·2010
Same author

Minimal external masculinization in a SRY-negative XX male Podenco dog.

Reproduction in domestic animals = Zuchthygiene·2008
Same author

Contacts between medial and lateral perforant pathway fibers and parvalbumin expressing neurons in the subiculum of the rat.

Neuroscience·2008

Area of Science:

  • Neuroscience
  • Cell Biology
  • Microscopy

Background:

  • Conventional light and fluorescence microscopy offer limited resolution for studying central nervous system fiber connectivity.
  • Determining precise fiber termination and synaptic connections requires higher resolution techniques.

Purpose of the Study:

  • To discuss methods for visualizing individual tracers in neural pathways.
  • To present techniques for visualizing dual markers to identify synaptic connections between specific neuronal elements.

Main Methods:

  • Review of existing and novel methods for visualizing neural tracers.
  • Development of dual-labeling strategies for electron microscopy.

Main Results:

  • Available methods allow visualization of individual tracers within neural circuits.

Related Experiment Videos

  • Dual-labeling approaches enable the identification of synaptic relationships between specific neuronal components.

    • Conclusions:

    • Electron microscopy is essential for confirming synaptic connectivity.
    • Advanced visualization techniques are crucial for detailed mapping of neural circuits.