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The genomic sequence of a contemporary wild-type mumps virus strain
1Enteric, Respiratory and Neurological Virus Laboratory, Central Public Health Laboratory, NW9 5HT, London, UK. ljin@phls.nhs.uk
Abstract:
Vaccination has the potential to eradicate mumps, and 82 countries now include a live attenuated mumps vaccine as part of their childhood vaccination programme. Although, monotypic, genetic variants of mumps virus (MuV) have been described based on comparison of the SH gene sequences, and at least seven genotypes have been identified. We now report the entire sequence of a recently isolated wild type MuV strain, Glouc1/UK96 (Glouc1) by direct sequencing of the cDNA obtained from cell culture fluid. The genome of this recent isolate was 15384 nucleotides in length. There were 579 nucleotide differences (3.8%) and 71 amino acid differences (1.5%) between Glouc1, a genotype G strain and Ur-AM9, a genotype B strain. Other MuV strains with available sequences were also compared with this pathological strain. The sequence of the contemporary strain reported here provides a picture of the variability of MuV over its entire genome (GenBank accession no. AF280799).
Insights
Researchers sequenced a wild type mumps virus (MuV) strain, revealing genetic variability. This mumps virus sequencing provides insights into viral evolution and aids in understanding vaccine effectiveness against diverse MuV genotypes.
Area of Science:
- Virology
- Genetics
Background:
- Mumps virus (MuV) exhibits genetic diversity with at least seven identified genotypes.
- Live attenuated mumps vaccines are part of childhood immunization programs in 82 countries, aiming for mumps eradication.
Purpose of the Study:
- To determine the complete genome sequence of a recently isolated wild-type mumps virus strain, Glouc1/UK96.
- To analyze the genetic variability of mumps virus over its entire genome.
Main Methods:
- Direct sequencing of cDNA derived from cell culture fluid of the Glouc1/UK96 mumps virus isolate.
- Comparative genomic analysis of the Glouc1 strain against other available mumps virus sequences.
Main Results:
- The complete genome of the Glouc1/UK96 mumps virus strain was sequenced, measuring 15,384 nucleotides.
- Significant genetic differences were observed between the genotype G strain (Glouc1) and a genotype B strain (Ur-AM9), with 3.8% nucleotide and 1.5% amino acid variations.
Conclusions:
- The full genome sequence of the contemporary mumps virus strain provides a comprehensive view of its genetic variability.
- Understanding mumps virus genetic diversity is crucial for monitoring viral evolution and assessing vaccine efficacy.