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Hepatocyte encapsulation for enhanced cellular functions.

S M Chia1, K W Leong, J Li

  • 1National University Medical Institutes, National University of Singapore, Singapore.

Tissue Engineering
|November 14, 2000
PubMed
Summary

Encapsulating rat hepatocytes in a novel two-layer membrane significantly enhances cellular functions for bioartificial liver devices. This innovative approach improves cell viability and function, crucial for treating acute liver failure.

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Area of Science:

  • Biomedical Engineering
  • Hepatology
  • Materials Science

Background:

  • Acute liver failure necessitates innovative treatments like bioartificial liver-assisted devices.
  • Hepatocyte encapsulation is a promising strategy for bioreactor design in liver support.
  • Optimizing cell function and immune protection within a bioreactor is critical.

Purpose of the Study:

  • To develop and evaluate a novel microencapsulation technique for rat hepatocytes.
  • To assess the functional enhancement and biocompatibility of encapsulated hepatocytes.
  • To determine the suitability of these microcapsules for bioartificial liver applications.

Main Methods:

  • Rat hepatocytes were encapsulated using complex coacervation into a two-layer polymeric membrane.

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  • The outer layer comprised a synthetic polymer (2-hydroxyethyl methacrylate, methacrylic acid, methyl methacrylate).
  • The inner layer consisted of modified collagen, and mechanical properties were assessed via nano-indentation.

    • Main Results:

    • Encapsulated hepatocytes exhibited cellular functions up to three times higher than monolayer controls.
    • The microcapsules allowed selective permeability for small molecules, facilitating nutrient and metabolite exchange.
    • The microcapsule structure prevented immune attack and showed no adverse collagen "skin effect".

    Conclusions:

    • The developed two-layer microcapsules effectively enhance hepatocyte function and viability.
    • These microcapsules are suitable for use in bioartificial liver-assisted devices.
    • This encapsulation method offers a promising strategy for improving liver support therapies.