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High-throughput expression of fusion proteins
M Nasoff1, M Bergseid, J P Hoeffler
1Genomics Institute of the Novartis Research Foundation, San Diego, California 92121-1125, USA.
Methods in Enzymology
|November 15, 2000
Summary
Epitope tagging facilitates the rapid identification and purification of functional recombinant plasmids for protein research. This method accelerates downstream applications like protein purification and immunolocalization studies.
Area of Science:
- Molecular Biology
- Biochemistry
- Protein Expression
Background:
- Fusion protein expression accelerates research.
- Epitope tags enable efficient identification of recombinant plasmids via Western blotting.
Purpose of the Study:
- To present protocols for utilizing epitope tags in molecular biology research.
- To demonstrate the utility of epitope tags in identifying functional plasmids.
Main Methods:
- Expression of open reading frames (ORFs) as fusion proteins.
- Utilizing Western blotting for identification of recombinant plasmids.
- Application of protocols to various expression vectors.
Main Results:
- Epitope tags streamline the identification of useful recombinant plasmids.
- Protocols are adaptable for high-throughput assays and downstream applications.
Conclusions:
- Epitope tagging is a versatile tool for protein research.
- The presented protocols are accessible and enhance protein expression studies.