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Related Experiment Videos

Stem cell collection using the dideco excel continuous flow blood cell separator: parameters for optimal stem cell

M Lai1, G Menichella, L Pierelli

  • 1Chair of Hematology, Università Cattolica del Sacro Cuore, Roma, Italy. mlai.w@tin.it

The International Journal of Artificial Organs
|November 15, 2000
PubMed
Summary

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This study shows the Dideco Excel separator effectively collects stem cells, with CD34+ cell counts predicting successful transplantation. The system efficiently harvests progenitor cells, ensuring prompt engraftment in most patients.

Area of Science:

  • Hematology
  • Transplantation Medicine
  • Cellular Therapy

Background:

  • Stem cell collection is crucial for transplantation.
  • The Dideco Excel separator is used for apheresis.
  • Evaluating collection efficiency and yield is important for patient outcomes.

Purpose of the Study:

  • To assess the efficacy of the Dideco Excel blood cell separator for stem cell collection.
  • To analyze stem cell yields and collection efficiencies in patients with hematological malignancies and solid tumors.
  • To determine factors influencing the collection of sufficient CD34+ cells for transplantation.

Main Methods:

  • Analysis of 55 apheresis procedures in 32 patients undergoing chemotherapy plus G-CSF mobilization.
  • Evaluation of blood volume processed, flow rate, and pre-collection cell counts (NC, MNC, CD34+).

Related Experiment Videos

  • Calculation of cell yields (NC, MNC, CD34+), yields per kilogram, collection efficiencies, and product contamination (RBC, platelets).
  • Statistical analysis including correlation and logistic regression to identify predictors of sufficient CD34+ cell collection.

    • Main Results:

    • The Dideco Excel system processed a median of 15.8 liters of blood per procedure.
    • Collection efficiencies were: NC 10.79%, MNC 29.06%, CD34+ 42.33%, PLT 26.5%.
    • Median CD34+ cell yield was 4.37x10^6 cells, with 42.33% collection efficiency.
    • CD34+ cell pre-counts strongly correlated with CD34+ yields/kg (r=0.82, p=0.000).
    • CD34+ pre-counts significantly influenced the collection of adequate CD34+ cells for transplantation (p=0.008).
    • 68.8% of patients received >2.5x10^6/kg CD34+ cells in a single procedure.

    Conclusions:

    • The Dideco Excel blood cell separator is effective for collecting progenitor cells.
    • Pre-collection CD34+ cell counts are a significant predictor of successful stem cell collection for transplantation.
    • The system facilitates prompt and lasting trilineage engraftment in transplanted patients.