Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Borrelia genomes in the year 2000.

S Casjens1

  • 1Department of Oncological Sciences, University of Utah Medical School, Salt Lake City 84132, USA. sherwood.casjens@hci.utah.edu

Journal of Molecular Microbiology and Biotechnology
|November 15, 2000
PubMed
Summary

The Borrelia burgdorferi B31 genome features a linear chromosome with essential genes and numerous plasmids. These plasmids, containing outer surface protein genes, show evidence of rearrangements and potential mutational decay.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methylation of L1RE1, RARB, and RASSF1 function as possible biomarkers for the differential diagnosis of lung cancer.

PloS one·2018
Same author

Effects of bioaerosol exposure on respiratory health in compost workers: a 13-year follow-up study.

Occupational and environmental medicine·2016
Same author

Prevalence of work-related rhino-conjunctivitis and respiratory symptoms among domestic waste collectors.

Advances in experimental medicine and biology·2014
Same author

Biomarker research with prospective study designs for the early detection of cancer.

Biochimica et biophysica acta·2013
Same author

A practical guide to epidemiological practice and standards in the identification and validation of diagnostic markers using a bladder cancer example.

Biochimica et biophysica acta·2013
Same author

Where are the pseudogenes in bacterial genomes?

Trends in microbiology·2002

Area of Science:

  • Microbiology
  • Genomics
  • Bacterial Pathogenesis

Background:

  • Borrelia species are spirochetes with a unique genetic makeup, including a linear chromosome and multiple plasmids.
  • Understanding the Borrelia burgdorferi B31 genome is crucial for comprehending its parasitic lifestyle and pathogenesis.

Purpose of the Study:

  • To analyze the complete genome sequence of Borrelia burgdorferi B31.
  • To characterize the genetic content and potential functions of its chromosome and plasmids.

Main Methods:

  • Whole-genome sequencing of Borrelia burgdorferi B31.
  • Bioinformatic analysis of chromosomal and plasmid DNA sequences.
  • Identification and prediction of gene functions, particularly for outer surface proteins.

Main Results:

  • The linear chromosome (approx. 910 kbp) contains most housekeeping genes, supporting a minimal metabolic capacity requiring host scavenging.
  • Multiple plasmids (linear and circular, 5-200 kbp) constitute a significant portion of the genome, encoding numerous predicted lipoproteins, many as outer surface proteins.
  • Ten linear plasmids exhibit low protein-coding potential and evidence of past duplicative rearrangements, with DNA undergoing mutational decay.

Conclusions:

  • The Borrelia burgdorferi B31 genome is characterized by a core chromosome and accessory plasmids crucial for its parasitic survival.
  • Plasmid-borne lipoproteins are likely key factors in host-pathogen interactions.
  • The observed plasmid DNA decay suggests evolutionary processes shaping the accessory genome.

Related Experiment Videos