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Development of shuttle vectors for spirochetes
I S Girons1, B Chi, H Kuramitsu
1Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France. isgirons@pasteur.fr
Journal of Molecular Microbiology and Biotechnology
|November 15, 2000
Abstract:
Constructions of Escherichia coli-spirochete shuttle vectors are based on naturally occurring plasmids, broad host range plasmids or bacteriophages. This review primarily focuses on genetic tools for Treponema denticola which is associated with periodontal diseases. The T. pallidum FlaA protein, E. coli beta-galactosidase, and the green fluorescent protein were successfully expressed in T. denticola from a shuttle vector system.