Modification of the acetylcholinesterase (AchE) staining method in Hirschsprung's disease
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Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
Published on: April 26, 2019
V Chentanez1, S Chittmittrapap, V Kasantikul
1Department of Anatomy, Chulalongkorn University, Bangkok, Thailand.
Journal of the Medical Association of Thailand = Chotmaihet Thangphaet
|November 15, 2000
Abstract:
Demonstration of the increasing activity of acetylcholinesterase (AChE) in a segment of the colon has proved to be the most accurate diagnostic tool to diagnose Hirschsprung's disease. Two methods of histochemical assessment were tried to establish the most appropriate and effective method for this study within the limitation of available equipment. Lake's method was chosen and was modified as the standard histochemical examination.
