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Suppressor mutation in Pseudomonas aeruginosa.

M Kageyama, Y Sano, T Shinomiya

    Journal of Bacteriology
    |June 1, 1979
    PubMed
    Summary
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    Researchers identified suppressor mutations in Pseudomonas aeruginosa, comparing them to Escherichia coli systems. These findings suggest Pseudomonas suppressors are likely amber suppressors, impacting phage propagation.

    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Suppressor mutations play a crucial role in genetic research by allowing the study of essential genes.
    • Understanding suppressor systems in different bacterial species is vital for comparative genomics and molecular biology.

    Purpose of the Study:

    • To identify and characterize suppressor mutations in Pseudomonas aeruginosa.
    • To compare the Pseudomonas aeruginosa suppressor systems with those found in Escherichia coli.
    • To investigate the propagation of suppressor-sensitive phages in Pseudomonas aeruginosa.

    Main Methods:

    • Isolation of suppressor-sensitive (sus) derivatives of plasmid RP4 trp and IncP1 plasmid-specific phages using E. coli.
    • Transfer of plasmid RP4 trp (sus) to P. aeruginosa strains and selection of Trp+ variants.

    Related Experiment Videos

  • Propagation of various suppressor-sensitive phages on P. aeruginosa suppressor-positive mutants and E. coli strains.
  • Comparison of phage plating efficiencies.

    • Main Results:

    • Identified suppressor-positive mutants in P. aeruginosa capable of propagating certain suppressor-sensitive phages.
    • Results suggest that the identified Pseudomonas suppressors are likely amber suppressors.
    • Some P. aeruginosa suppressors could propagate amber-type phages equally well in both suppressed and non-suppressed strains.
    • Certain phage mutants suppressed in E. coli were not suppressed by the P. aeruginosa suppressor.

    Conclusions:

    • Pseudomonas aeruginosa possesses functional suppressor systems, likely amber suppressors.
    • The identified suppressors exhibit some differences compared to E. coli suppressor systems, particularly in phage propagation.
    • Further characterization of P. aeruginosa bacteriophages E79 and D3 revealed additional suppressor-sensitive mutants.