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Related Experiment Videos

The WNT antagonist cSFRP2 modulates programmed cell death in the developing hindbrain.

D L Ellies1, V Church, P Francis-West

  • 1MRC Centre for Developmental Neurobiology, King's College London, Guy's Campus, London, SE1 1UL, UK.

Development (Cambridge, England)
|November 15, 2000
PubMed
Summary

Programmed cell death in avian hindbrain neural crest is regulated by WNT and BMP signaling. Pre-patterned cSFRP2 expression controls cell death, influencing crest cell patterning.

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Area of Science:

  • Developmental biology
  • Neuroscience
  • Cell biology

Background:

  • Premigratory neural crest cells in the avian hindbrain undergo programmed cell death.
  • This cell death in rhombomeres 3 and 5 (r3, r5) is crucial for patterning emigrant crest cells into three streams.
  • Upregulation of Bmp4 and Msx2 in r3 and r5 induces this programmed cell death.

Purpose of the Study:

  • To investigate the role of cSFRP2 in regulating programmed cell death in the avian hindbrain.
  • To elucidate the molecular mechanisms underlying neural crest cell patterning via cell death.
  • To understand the interplay between WNT and BMP signaling in this process.

Main Methods:

  • Overexpression of cSfrp2 in r3 and r5.
  • Depletion of cSFRP2 function in r4.

Related Experiment Videos

  • Analysis of Bmp4 and Msx2 expression levels.
  • Assessment of programmed cell death incidence.

    • Main Results:

    • Overexpression of cSfrp2 inhibited Bmp4 expression and prevented programmed cell death in r3 and r5.
    • Depletion of cSFRP2 in r4 led to increased Msx2 expression and ectopic programmed cell death.
    • Wnt1 overexpression also induced ectopic programmed cell death.

    Conclusions:

    • Pre-patterned cSFRP2 expression modulates programmed cell death in the rhombencephalic neural crest.
    • A WNT-BMP signaling loop is proposed to regulate this cell death and subsequent crest cell patterning.
    • This study reveals a novel regulatory mechanism in neural crest development.