Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Replication of Semliki Forest virus.

L Kääriäinen, S Keränen, B Lachmi

    Medical Biology
    |October 1, 1975
    PubMed
    Summary

    Semliki Forest virus replicates in cell cytoplasm, producing viral RNA and proteins. A temperature-sensitive mutant reveals translational control mechanisms affecting nonstructural protein synthesis during alphavirus replication.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Diffusion tensor imaging and tractography of the white matter in normal aging: The rate-of-change differs between segments within tracts.

    Magnetic resonance imaging·2017
    Same author

    The freezing method of cleavage stage embryos has no impact on the weight of the newborns.

    Journal of assisted reproduction and genetics·2016
    Same author

    Lobar lung resection in elderly patients with non-small cell lung carcinoma: impact of cardiac comorbidity on surgical outcome.

    Swiss medical weekly·2013
    Same author

    Viral RNA replication in association with cellular membranes.

    Current topics in microbiology and immunology·2004
    Same author

    Cerebral atrophy as predictor of cognitive function in old, community-dwelling individuals.

    Acta neurologica Scandinavica·2004
    Same author

    Model-based mutagenesis to improve the enantioselective fractionation properties of an antibody.

    Protein engineering·2004

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Semliki Forest virus (SFV), an alphavirus, replicates in the cytoplasm of eukaryotic cells.
    • Viral RNA (42S RNA) directs the synthesis of RNA-dependent RNA polymerases.
    • These polymerases create complementary 45S RNA, serving as a template for 45S and 26S positive RNA strands.

    Purpose of the Study:

    • To investigate the replication cycle of Semliki Forest virus.
    • To understand the translational control mechanisms governing viral protein synthesis.
    • To analyze the role of a temperature-sensitive mutant (ts-1) in nonstructural protein production.

    Main Methods:

    • Analysis of RNA synthesis and sedimentation values (45S and 26S).
    • Investigation of polysome association with viral RNAs.
    • Characterization of protein synthesis using a temperature-sensitive mutant (ts-1) at nonpermissive temperatures.

    Main Results:

    • BHK cells produce approximately 200,000 molecules of each RNA species per cell.
    • 26S RNA codes for viral structural proteins (capsid, E2, E1) translated as a polyprotein.
    • The ts-1 mutant synthesizes nonstructural proteins (78,000 and 86,000 MW) at nonpermissive temperatures, indicating translational control.
    • Viral nucleocapsid assembly involves capsid protein binding to 42S RNA, followed by budding through the plasma membrane.

    Conclusions:

    • Semliki Forest virus replication involves distinct RNA species and translational regulation.
    • The ts-1 mutant provides insights into the control of nonstructural protein synthesis.
    • Viral assembly and release are efficient processes, with significant RNA and protein production per cell.

    Related Experiment Videos