Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer.

K Shibata1, M Itoh, K Aizawa

  • 1Genome Exploration Research Group, RIKEN, Genomic Sciences Center (GSC) and Genome Science Laboratory, RIKEN, Tsukuba Institute, Core Research of Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), Ibaraki, Japan.

Genome Research
|November 15, 2000
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of Muscle Strength and Gait Speed with Cross-Sectional Muscle Area Determined by Mid-Thigh Computed Tomography - A Comparison with Skeletal Muscle Mass Measured by Dual-Energy X-Ray Absorptiometry.

The Journal of frailty & aging·2020
Same author

Gastrointestinal: Burkitt lymphoma showing multiple tumorous lesions in the gastrointestinal tract.

Journal of gastroenterology and hepatology·2019
Same author

Gastrointestinal: Multiple venous malformations and polyps of the small intestine in Cowden syndrome.

Journal of gastroenterology and hepatology·2018
Same author

Roles of histone H3.5 in human spermatogenesis and spermatogenic disorders.

Andrology·2017
Same author

A New Grip Strength Measuring Device for Detailed Evaluation of Muscle Contraction among the Elderly.

The Journal of frailty & aging·2016
Same author

Topic: Incisional Hernia - "Easy case" as daily case: open vs lap, where the mesh, which fixation…in center midline cases.

Hernia : the journal of hernias and abdominal wall surgery·2015
This summary is machine-generated.

The RIKEN high-throughput sequencing pipeline (RISA system) enables rapid, accurate DNA sequencing for large-scale projects like the mouse encyclopedia, processing 50,000 samples daily.

Area of Science:

  • Genomics
  • Biotechnology
  • High-throughput screening

Background:

  • The RIKEN mouse encyclopedia project requires efficient, large-scale DNA sequencing.
  • Existing sequencing technologies may not meet the throughput demands for comprehensive genomic studies.

Purpose of the Study:

  • To develop a novel high-throughput 384-format capillary sequencing system (RISA system) for large-scale genomic projects.
  • To enhance DNA sequencing efficiency and accuracy for applications such as the RIKEN mouse encyclopedia.

Main Methods:

  • Development of a 384-multicapillary auto sequencer (RISA sequencer) with a scanning optical system.
  • Integration of automated colony picking, template preparation, and sequencing reaction components.
  • Utilization of fluorescent-labeled sequencing reactions compatible with transcriptional and cycle sequencing.

Related Experiment Videos

Main Results:

  • The RISA system processes 50,000 DNA samples per day using 16 RISA sequencers.
  • Achieved average read length of 654.4 bp with >99% accuracy for long-read sequencing.
  • Analyzed 384 samples in 1.5 hours for short-read sequencing (350 bp).

Conclusions:

  • The RISA system significantly advances high-throughput DNA sequencing capabilities.
  • Enables rapid whole-genome sequencing of higher organisms within a month.
  • Provides a robust platform for large-scale genomic initiatives and biological research.