Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

p300 does not require its acetylase activity to stimulate p73 function.

X Zeng1, H Lee, Q Zhang

  • 1Department of Biochemistry and Molecular Biology, Oregon Health Science University, Portland, Oregon 97201, USA.

The Journal of Biological Chemistry
|November 15, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic variation of herpesvirus saimiri subgroup A transforming protein and its association with cellular src.

Journal of virology·1997
Same author

[Efficacy of MR angiographic original images on surgery for posterior communicating artery aneurysms].

No shinkei geka. Neurological surgery·1997
Same author

A high-risk group for prostatism: a population-based epidemiological study in Korea.

British journal of urology·1997
Same author

Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase.

Journal of virology·1997
Same author

Streptomyces seoulensis sp. nov.

International journal of systematic bacteriology·1997
Same author

Histamine inhibits ATP-induced [Ca2+]i rise through the activation of protein kinase A in HL-60 cells.

European journal of pharmacology·1997
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
See all related articles

The coactivator p300 (also known as cAMP-response element-binding protein-binding protein) does not require its acetylase activity to enhance p73 protein function. This finding clarifies the mechanism of p73 transcriptional regulation.

Area of Science:

  • Molecular Biology
  • Protein Biochemistry
  • Gene Regulation

Background:

  • p73, a tumor suppressor, functions similarly to p53.
  • p53 and p73 utilize coactivators like p300 (cAMP-response element-binding protein-binding protein).

Purpose of the Study:

  • To investigate if the intrinsic acetylase activity of p300 is essential for its role as a p73 coactivator.
  • To elucidate the mechanism by which p300 modulates p73 function.

Main Methods:

  • In vitro acetylation assays using p73 fragments and full-length p73.
  • In vivo co-expression and acetylation studies in cells.
  • Functional assays using an acetylase-defective p300 mutant (p300AT2).
  • Analysis of p300-p73 protein-protein interactions and p73 protein stability.

Related Experiment Videos

Main Results:

  • p300 efficiently acetylated a C-terminal fragment of p73 in vitro but not full-length p73 in vitro or in vivo.
  • An acetylase-defective p300 mutant (p300AT2) could still enhance p73-dependent transcription.
  • p300 associated with p73 within DNA-protein complexes.
  • p300 binding stabilized p73 proteins.

Conclusions:

  • The acetylase activity of p300 is not required for its function as a p73 coactivator.
  • p300 acts as a coactivator for p73 through mechanisms independent of its enzymatic acetylase function, likely involving protein stabilization and complex formation.