Related Experiment Videos
[Fibrillar actin promotes assembly of dissociated 20S-proteasome complex]
V E Galkin1, V N Babakov, L V Turoverova
1Institute of Cytology RAS, St. Petersburg.
Tsitologiia
|November 15, 2000
Abstract:
20S-proteasome was purified from rat liver cells by ultracentrifugation, gel-chromatography and ultrafiltration. The ability of 20S-proteasome to interact with fibrillar actin (F-actin) was examined by rapid cosidementation of these dissociated particles with F-actin in isokinetic sucrose gradient. Proteasomes, which were dissociated with Zn2+ ions, can be assembled on the fibrillar actin once again (with the exception of protein 27 kDa) at deleting ions Zn2+ from the solution with the help of EDTA.