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The establishment of a human liver nuclei two-dimensional electrophoresis reference map
E Jung1, C Hoogland, D Chiappe
1Central Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland. eva.jung@dim.hcuge.ch
Electrophoresis
|November 18, 2000
Abstract:
This short communication describes the establishment of a two-dimensional electrophoresis (2-DE) reference map of nuclear proteins isolated from human liver. The human liver nuclei 2-DE reference map contains 1497 spots. In an initial identification study using peptide mass fingerprinting as a means of protein identification we were able to identify 26 spots corresponding to 15 different proteins. The human liver nuclei 2-DE reference map is now included in the SWISS-2DPAGE database, which can be accessed through the ExPASy server (http://www.expasy.ch/ch2d/).