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Influence of age and K, Mg aspartate (Cardilan) on murine haemopoiesis
P Fedorocko1, N O Macková, Z Sándorcínová
1Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. Safárik University, Moyzesova 11, 04167, Kosice, Slovak Republic. fedvox@kosice.upjs.sk
Insights
Potassium and magnesium aspartate (Cardilan) supplementation positively impacts aging mice
Area of Science:
- Hematology
- Gerontology
- Nutritional Science
Background:
- Aging impacts hematopoietic stem cell function and peripheral blood cell counts.
- The effects of mineral aspartate supplementation on aging hematopoiesis are not well understood.
Purpose of the Study:
- To investigate the impact of aging on hematopoiesis in ICR mice.
- To evaluate the effects of short-term and long-term Cardilan (K and Mg salts of aspartic acid) intake on hematopoietic parameters in aging mice.
Main Methods:
- Assessed bone marrow cellularity, colony-forming cells (GM-CFC), spleen colony-forming units (CFU-S), and erythroid burst-forming units (BFU-E) in aging mice.
- Administered Cardilan via short-term and long-term drinking protocols to different age groups of mice.
- Monitored peripheral blood cell counts, including leukocytes and reticulocytes.
Main Results:
- Aging increased bone marrow CFU-S and BFU-E, while decreasing peripheral leukocytes.
- Short-term Cardilan intake boosted CFU-S, BFU-E, and reticulocytes in younger aging mice and increased spleen weight/cellularity and peripheral cells in older mice.
- Long-term Cardilan intake elevated spleen GM-CFC in younger aging mice and reticulocytes in older mice.
Conclusions:
- K and Mg salts of aspartic acid demonstrate a significant influence on erythropoietic activity.
- Cardilan supplementation may offer benefits for hematopoietic function during the aging process.
Abstract:
We have studied the effect of age as well as the effect of short-term and long-term intake of K and Mg salts of aspartic acid (Cardilan) on haemopoiesis in ICR mice strain. The cellularity of the bone marrow does not change with aging, but the number of granulocyte-macrophage colony-forming cells (GM-CFC) and also the number of spleen colony-forming units (CFU-S) and erythroid burst-forming units (BFU-E) in two-year-old mice increased in the bone marrow. In two-year-old mice the number of leukocytes decreased in the peripheral blood with aging, mainly as a result of a decrease in mononuclear cells. Short-term drinking (STD) of Cardilan caused increased numbers of CFU-S and BFU-E in bone marrow and increased numbers of reticulocytes in the peripheral blood of one-year old animals (STD/12 months old). In the oldest mice (STD/24) increased weight and cellularity of the spleen and rapid increase of leukocytes and reticulocytes in the peripheral blood was recorded. After long-term drinking (LTD) of Cardilan the number of spleen GM-CFC rose markedly in one-year-old mice (LTD/12) and in two-year-old mice (LTD/24) the number of reticulocytes in the peripheral blood rose. Our results indicate that K and Mg salts of aspartic acid influence erythropoietic activity most widely.