Characterization of CAX-like genes in plants: implications for functional diversity

Transporter-mediated Ca(2+) efflux from the cytoplasm is an important component of plant signal transduction. To elucidate the diversity and role of Ca(2+)/H(+) in controlling plant cytosolic Ca(2+) concentrations, homologs of CAX (for calcium exchanger) genes were cloned from Zea mays and Arabidopsis thaliana cDNA libraries. The A. thaliana homolog of CAX (AtHCX1) is 77% identical to CAX1 while the Z. mays homolog of CAX (ZmHCX1) is 64% identical to CAX1 in amino acid sequence. AtHCX1 transcripts appeared to be expressed in all tissues, and levels of AtHCX1 RNA increased after Ca(2+) or Na(+) treatment. When expressed in yeast mutants defective in vacuolar Ca(2+) uptake, ZmHCX1 and AtHCX1 failed to suppress the Ca(2+) sensitivity of these strains. These results imply that CAX-like genes may have functions in plant ion homeostasis that differ from those of previously characterized CAX genes.