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Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
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Published on: January 10, 2011

Identifying the Ca(++) signalling sources activating chloride currents in Xenopus oocytes using ionomycin and

C L Thurman1, J S Burns, R G O'Neil

  • 1Department of Biology, University of Northern Iowa, Cedar Falls, IA 50614-0421, USA. Thurman@uni.edu

Cellular Signalling
|November 18, 2000
PubMed

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Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...