Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro and human testing strategies for skin irritation.

M K Robinson1, R Osborne, M A Perkins

  • 1The Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45253-8707, USA. robinson.mk@pg.com

Annals of the New York Academy of Sciences
|November 18, 2000
PubMed
Summary

Developing alternative testing methods for skin toxicity is crucial for worker and consumer safety. In vitro and human patch tests are effective replacements for animal testing in assessing skin irritation and corrosion.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding pregnant women's health literacy strengths and challenges: a cluster-based exploration in the Health Literacy in Pregnancy (HeLP) study.

Public health·2025
Same author

Results of a nationally implemented cardiac screening programme in elite cricket players in England and Wales.

Journal of science and medicine in sport·2022
Same author

Bariatric surgery and pain outcomes in osteoarthritis.

Osteoarthritis and cartilage·2021
Same author

Health literacy: a new piece of the puzzle in psoriasis care? A cross-sectional study.

The British journal of dermatology·2019
Same author

Sensitivity of the early-life stages of freshwater mollusks to neonicotinoid and butenolide insecticides.

Environmental pollution (Barking, Essex : 1987)·2016
Same author

Roz osborne on the support for the student bill of rights.

Nursing standard (Royal College of Nursing (Great Britain) : 1987)·2016

Area of Science:

  • Toxicology
  • Dermatology
  • In Vitro Testing

Background:

  • Assessing chemical and product skin toxicity (corrosion/irritation) is vital for safety.
  • Traditional animal testing faces ethical opposition and regulatory scrutiny.
  • Balancing human safety, regulatory compliance, and reduced animal use drives innovation.

Purpose of the Study:

  • To evaluate alternative, predictive methods for assessing skin toxicity.
  • To demonstrate the efficacy of in vitro and human-based tests as replacements for animal testing.
  • To support the reduction and eventual elimination of animal testing in regulatory safety assessments.

Main Methods:

  • Development and validation of in vitro skin corrosion assays using skin/epidermal equivalents.
  • Modification of in vitro assays to assess skin irritation potential against human data.

Related Experiment Videos

  • Implementation of a novel human patch test for acute skin irritation assessment.

    • Main Results:

    • In vitro skin/epidermal equivalent assays successfully distinguish corrosive from non-corrosive chemicals.
    • Strong correlation observed between in vitro assay data and human skin irritation data for chemicals and products.
    • Human patch tests offer a viable alternative for acute skin irritation assessment.

    Conclusions:

    • Validated in vitro and human-based methods can accurately assess skin corrosion and irritation potential.
    • These alternative strategies effectively replace traditional animal testing methods.
    • Current strategies enable adequate assessment of human skin irritation without animal use.