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Transient modulation of gene expression in the neurulation staged mouse embryo.

E S Hunter1, P Hartig

  • 1Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA. Hunter.sid@epa.gov

Annals of the New York Academy of Sciences
|November 18, 2000
PubMed
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Viral vectors transiently modulate gene expression in embryos for studying gene function during organogenesis. These tools enable gain- and loss-of-function models, aiding developmental biology research.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Understanding gene function during embryonic development is crucial.
  • Existing methods for gene modulation have limitations.

Purpose of the Study:

  • To explore the use of viral vectors for transient gene expression modulation in embryos.
  • To establish methods for studying gene function during early organogenesis.

Main Methods:

  • Adenoviral transduction of whole embryos, embryonic heart, vasculature, and neural crest cells.
  • Creation of gain-of-function and loss-of-function models.
  • Evaluation of morphological consequences of gene expression changes.

Main Results:

  • Demonstrated successful transient modulation of gene expression using adenoviral vectors.

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  • Enabled assessment of gene function through overexpression and knockdown.
  • Facilitated gene-teratogen interaction studies.

    • Conclusions:

    • Viral vectors are effective tools for transient gene expression modulation in embryonic studies.
    • These methods hold significant promise for advancing research in developmental biology and genetics.