Related Experiment Videos
Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum
R Durand1, J Eslahpazire, S Jafari
1Centre National de Référence pour la Chimiosensibilité du Paludisme, Université Paris V et Assistance Publique-Hôpitaux de Paris, Hôpital Bichat-Claude Bernard, Laboratoire de Parasitologie, Paris, France. rjdurand@club-internet.fr
Antimicrobial Agents and Chemotherapy
|November 18, 2000
Abstract:
A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.