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Related Experiment Videos

Vibrio cholerae requires rpoS for efficient intestinal colonization.

D S Merrell1, A D Tischler, S H Lee

  • 1Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

Infection and Immunity
|November 18, 2000
PubMed
Summary

The alternative sigma factor RpoS is crucial for Vibrio cholerae intestinal colonization in mice. Mutations in rpoS significantly reduced colonization, highlighting RpoS

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Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Vibrio cholerae is an intestinal pathogen found in aquatic environments.
  • Vibrio cholerae causes diarrheal disease through intestinal colonization in animal models.
  • The role of specific regulatory factors in V. cholerae colonization is not fully understood.

Purpose of the Study:

  • To investigate the role of the alternative sigma factor RpoS in Vibrio cholerae intestinal colonization.
  • To determine if RpoS is essential for V. cholerae pathogenesis in a suckling mouse model.

Main Methods:

  • Construction of independent mutations within the rpoS gene.
  • Assessment of V. cholerae colonization ability using competition assays in suckling mice.
  • Evaluation of in vitro growth characteristics of mutant strains.

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  • Analysis of spontaneous suppressor mutations.
  • Complementation of mutant phenotypes through trans-acting rpoS expression or allelic reversion.

    • Main Results:

    • Multiple independent rpoS mutations resulted in a fivefold decrease in V. cholerae intestinal colonization.
    • rpoS mutations did not affect in vitro growth in rich medium.
    • Spontaneous suppressor mutations were ruled out as a confounding factor.
    • Complementation studies confirmed that the reduced colonization was due to loss of functional RpoS.

    Conclusions:

    • The alternative sigma factor RpoS plays a significant role in Vibrio cholerae intestinal colonization.
    • RpoS is essential for the colonization fitness of V. cholerae in the suckling mouse model.
    • These findings identify RpoS as a key factor in V. cholerae pathogenesis.