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Updated: Jul 12, 2026

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
Published on: October 23, 2016
Lipids, lipid modification and lipid-protein interaction in membrane budding and fission--insights from the roles of
1Department of Neurobiology, Interdisciplinary Center of Neuroscience, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany. whuttner@sun0.urz.uni-heidelberg.de
Abstract:
Studies on the endocytosis of synaptic vesicles have provided two novel insights into the mechanism of vesicle formation from donor membranes, both of which concern lipids. One is the essential role of endophilin, a cytosolic protein converting lysophosphatidic acid by addition of the fatty acid arachidonate into phosphatidic acid. The other is the essential role of membrane cholesterol, which specifically interacts with synaptophysin, the major transmembrane protein of synaptic vesicles. These findings reveal novel modes of membrane lipid modification and lipid-protein interaction in vesicle biogenesis.
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