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Electrophoresis-based fluorescent dideoxy-terminator sequencing
1Applied Biosystems, Foster City, CA 94070, USA.
Novartis Foundation Symposium
|November 21, 2000
Summary
Electrophoretic DNA sequencing technology continues to advance, ensuring its leading role in genetic analysis. Ongoing improvements will adapt this powerful tool for both large-scale and routine gene sequencing needs.
Area of Science:
- Genetics
- Biotechnology
- Molecular Biology
Background:
- Electrophoretic DNA sequencing has been a foundational technology for over a decade.
- Continuous innovation is crucial for maintaining its relevance in genetic analysis.
Purpose of the Study:
- To highlight the enduring importance and future potential of electrophoretic DNA sequencing.
- To emphasize the technology's adaptability to evolving research demands.
Main Methods:
- Advances in separation science.
- Improvements in detection methodologies.
- Enhanced automation and sample preparation techniques.
Main Results:
- Electrophoretic technology remains at the forefront of genetic analysis.
- The technology is evolving to meet new demands in sequencing.
Conclusions:
- Electrophoretic DNA sequencing technology will continue to adapt and serve the scientific and commercial communities.
- Future applications will range from de novo whole genome analysis to comparative sequencing of known genes.