[Data on the biological characteristics of serological group O4 Escherichia isolated in acute intestinal diseases]
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Abstract:
A study was made of 155 strains of E. coli of the O4 serological group isolated from sick children and adults during group and sporadic acute intestinal diseases), from persons who came in contact with them, and also from healthy persons during prophylactic examination; three standard cultures were examined as well. Along with strains with a typical enzymatic activity there were strains which produced retarded lactose fermentation and also gas-free, immobile and lysin-negative strains resembling Shigellae. Eight biochemical types were determined among E. coli 04. A study of the antigenic structure by cross agglutinin adsorption indicated identicity of the strains by O-antigen and their difference by the K- and H-antigens. Circulation of E. coli of serological types O4: K12(L): H1,O4: K3(L): H5,O4: K3(L): H12,04: K12(L): H40,04: K52(L): H4, and O4: K12(L) HII was revealed; the first two serological types prevailed. Serological types of O4: K3(L):H12, O4: K12(L): HI and O4: K12(L): H40, isolated in cases of group and sporadic acute intestinal diseases were described for the first time.
Insights
This study investigated Escherichia coli O4 strains from various sources, identifying eight biochemical types and specific serological profiles. Certain E. coli O4 serotypes were newly described in acute intestinal disease outbreaks.
Area of Science:
- Microbiology
- Immunology
- Epidemiology
Background:
- Escherichia coli (E. coli) is a common bacterium.
- The O4 serological group of E. coli can be associated with intestinal diseases.
- Understanding E. coli O4 diversity is crucial for public health.
Purpose of the Study:
- To characterize the biochemical and antigenic properties of E. coli O4 strains.
- To identify circulating serotypes of E. coli O4 in human infections and contacts.
- To describe novel serotypes associated with acute intestinal diseases.
Main Methods:
- Isolation and cultivation of 155 E. coli O4 strains from clinical and healthy individuals.
- Biochemical testing for enzymatic activity, lactose fermentation, motility, and lysin production.
- Serological analysis using cross-agglutinin adsorption to determine O, K, and H antigen profiles.
Main Results:
- Eight distinct biochemical types of E. coli O4 were identified.
- Strains exhibited variations in lactose fermentation, motility, and lysin production.
- Identical O-antigens but diverse K- and H-antigens were observed among strains.
- Six E. coli O4 serotypes were identified, with O4:K12(L):H1 and O4:K3(L):H5 being predominant.
- Three serotypes (O4:K3(L):H12, O4:K12(L):HI, and O4:K12(L):H40) were newly described in acute intestinal disease cases.
Conclusions:
- E. coli O4 demonstrates significant biochemical and antigenic diversity.
- Specific E. coli O4 serotypes are associated with acute intestinal diseases.
- The findings contribute to the epidemiological understanding of E. coli O4 infections.