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Published on: November 15, 2013
The yeast two-hybrid system and its pharmaceutical significance
1Department of Pharmaceutical Biotechnology, School of Pharmacy, Ege University, Izmir, Turkey. ztopcu@bornova.ege.edu.tr
Abstract:
The detected phenotypes in many diseases are caused from dysfunction in protein-protein, protein-DNA and receptor-ligand interactions. Therefore, determination of these molecular interactions followed by designing or screening the compounds to target these interactions provides a significant challenge in drug development. This review aims to highlight the yeast two-hybrid system in determination of protein-protein interactions and its possible outcomes in pharmaceutical research. The variations of the basic methodology as one- and three-hybrid systems are also discussed in relation to their potential pharmaceutical applications.
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