Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Proliferation pattern changes in the zebrafish brain from embryonic through early postembryonic stages.

M F Wullimann¹, S Knipp

¹Brain Research Institute, University of Bremen, Germany. wullimann@uni-bremen.de

Anatomy and Embryology

|November 23, 2000

Summary

Proliferating cell nuclear antigen (PCNA) shows brain development in zebrafish. Proliferation zones become restricted over time, with distinct patterns in hindbrain and forebrain.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

'Cloudbuster': a Python-based open source application for three-dimensional reconstruction and quantification of stacked biological imaging samples.

Interface focus·2022

Same author

Steroid-responsive cauda equina syndrome associated with GVHD after allogeneic hematopoietic stem cell transplantation.

Bone marrow transplantation·2007

Same author

A pilot study of bendamustine in elderly patients with high-risk MDS and AML.

Leukemia & lymphoma·2007

Same author

Pitfall in coronary artery bypass surgery: poor flow of left internal mammary artery to left anterior descending artery graft due to compression by a chest drain.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery·2002

Same author

[Evaluation of myocardial viability with contrast-enhanced magnetic resonance imaging--comparison of the late enhancement technique with positronemission tomography].

RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin·2002

Same author

Detailed immunohistology of Pax6 protein and tyrosine hydroxylase in the early zebrafish brain suggests role of Pax6 gene in development of dopaminergic diencephalic neurons.

Brain research. Developmental brain research·2001

Area of Science:

Neuroscience
Developmental Biology
Zebrafish Research

Background:

Understanding brain development is crucial for neuroscience.
Zebrafish (Danio rerio) are a key model organism for studying vertebrate development.
Proliferation patterns guide neurogenesis and brain structure formation.

Purpose of the Study:

To map the spatiotemporal course of cell proliferation in the developing zebrafish brain.
To identify specific proliferation zones and their dynamics during embryonic and postembryonic stages.
To correlate proliferation patterns with neuroanatomical landmarks.

Main Methods:

Proliferating cell nuclear antigen (PCNA)-immunohistochemistry was employed.
Combined Bodian fiber silver-stain/cresyl Nissl-stain facilitated morphogenetic analysis.

Related Experiment Videos

Zebrafish brains were analyzed from embryonic (24 h) to postembryonic (5 days) stages.

Main Results:

Initially ubiquitous proliferation in the neural tube becomes restricted to specific gray matter cells by 48-72 hours.
Segmental organization of proliferation zones observed in hindbrain and forebrain.
Rhombomeric pattern emerges earlier than prosomeric pattern; midbrain-hindbrain boundary shows sustained proliferation.

Conclusions:

Zebrafish brain development involves dynamic and spatially regulated proliferation zones.
Distinct temporal and spatial patterns of proliferation underlie the formation of brain structures.
The midbrain-hindbrain boundary is a region of sustained proliferative activity.