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An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
Published on: November 3, 2010
A method for the simulation of normal, carrier and affected controls for PCR-RFLP screening of a genetic disease in
P N Mukhopadhyaya1, H H Mehta, R N Rathod
1Biotechnology Laboratory, National Dairy Development Board, Anand-388 001, Gujarat, India. pratapm@anand.nddb.ernet.in
Molecular and Cellular Probes
|November 25, 2000
Abstract:
A technique is described that may be used to create in vitro mutations in PCR templates to generate affected and carrier controls for diagnostic testing when DNA from such individuals is not easily obtained. The method is demonstrated for a PCR-RFLP diagnostic test of the genetic disorder BLAD (Bovine Leukocyte Adhesion Deficiency).
