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Updated: Jul 12, 2026
06:38
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
Epstein-Barr virus and gastric carcinoma
1Department of Tumor Virology, Hokkaido University, Sapporo, Japan. kentaka@med.hokudai.ac.jp
Molecular Pathology : MP
|November 25, 2000
Summary
Epstein-Barr virus (EBV) drives gastric carcinoma development by infecting single cells. EBV immortalizes gastric cells, promoting malignant properties and supporting the progression of EBV-positive gastric cancer.
Area of Science:
- Oncology
- Virology
- Molecular Biology
Background:
- Epstein-Barr virus (EBV) is found in approximately 10% of gastric carcinoma cases globally.
- EBV infection is consistently present in 100% of carcinoma cells within these cases.
- EBV's role in epithelial malignancies is difficult to study due to epithelial cells' in vitro resistance to infection.
Purpose of the Study:
- To investigate the role of Epstein-Barr virus (EBV) in the development and progression of EBV-positive gastric carcinomas.
- To overcome the challenge of in vitro EBV infection in epithelial cells.
- To determine if EBV infection can induce malignant transformation in primary gastric epithelial cells.
Main Methods:
- Utilized recombinant EBV with a selectable marker to enable infection of epithelial cells.
- Established EBV-infected cell clones from carcinoma cell lines, identifying cell-to-cell contact as an efficient infection mode.
- Infected primary gastric epithelial cells with EBV and assessed their properties in vitro and in vivo.
Main Results:
- Successfully immortalized primary gastric epithelial cells via EBV infection.
- Infected cells expressed characteristic EBV genes (EBNA1, EBER1, EBER2, BARF0, and sometimes LMP2A) found in EBV-positive gastric carcinoma.
- EBV-infected gastric epithelial cells exhibited accelerated malignant characteristics, including soft agarose growth and tumorigenicity in SCID mice.
Conclusions:
- EBV plays a significant role in the initiation and development of EBV-positive gastric carcinomas.
- EBV infection can induce malignant transformation of primary gastric epithelial cells.
- EBV contributes to maintaining the malignant phenotype of established EBV-positive gastric carcinoma.
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