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A sequence-ready map for human chromosome 12q15-21.
1Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|November 25, 2000
Summary
Researchers created a sequence-ready clone map for a human chromosome 12 region amplified in liposarcoma. This bacterial artificial chromosome map aids in sequencing and gene mapping of this specific genomic segment.
Area of Science:
- Genomics and Bioinformatics
- Molecular Biology
- Cancer Research
Background:
- Human genome sequencing requires accurate clone maps.
- A specific region on chromosome 12q15-21 is frequently amplified in liposarcoma.
- Previous maps relied on Yeast Artificial Chromosomes (YACs) and framework maps.
Purpose of the Study:
- To construct a sequence-ready Physical map of bacterial clones (PAC/BAC) for a liposarcoma-amplified region on chromosome 12.
- To integrate existing markers and develop new ones for high-resolution mapping.
- To provide a framework for complete DNA sequencing and gene mapping of the target region.
Main Methods:
- Screened PAC and BAC libraries using 22 non-polymorphic Sequence Tagged Site (STS) markers.
- Assembled bacterial clones into contigs based on marker content.
- Extended contigs via end sequencing, generating new STSs and iterative library screening.
Main Results:
- Constructed 5 contigs covering 3.6 Mb of chromosome 12q15-21.
- The map comprises 17 YACs, 187 PACs, 160 BACs, and 17 cosmids.
- Placed 131 markers (including 97 newly developed) with an average resolution of approximately 28 kb.
Conclusions:
- A high-resolution, sequence-ready clone contig map was successfully built for a clinically relevant genomic region.
- This map serves as a crucial framework for future DNA sequencing and gene identification efforts.
- The developed map enhances our understanding of the genomic landscape in liposarcoma.