Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple roles for Gata5 in zebrafish endoderm formation.

J F Reiter1, Y Kikuchi, D Y Stainier

  • 1Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, University of California, San Francisco, San Francisco, CA 94143-0448, USA.

Development (Cambridge, England)
|November 28, 2000
PubMed
Summary

Gata5 is crucial for zebrafish endoderm development, regulating cell numbers and gene expression in organs like the liver and pancreas. It works with other factors in a key developmental pathway.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endoderm specification and liver development.

Methods in cell biology·2016
Same author

The zebrafish as a model system to study cardiovascular development.

Trends in cardiovascular medicine·2011
Same author

Endocardial cushion formation in zebrafish.

Cold Spring Harbor symposia on quantitative biology·2003
Same author

Positional cloning of heart and soul reveals multiple roles for PKC lambda in zebrafish organogenesis.

Current biology : CB·2001
Same author

UDP-glucose dehydrogenase required for cardiac valve formation in zebrafish.

Science (New York, N.Y.)·2001
Same author

casanova encodes a novel Sox-related protein necessary and sufficient for early endoderm formation in zebrafish.

Genes & development·2001

Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Zebrafish Model Organisms

Background:

  • Gata5 (zinc-finger transcription factor) is known to be essential for zebrafish gut tube development.
  • Endodermal organs are critical for organismal function and development.

Purpose of the Study:

  • To investigate the role of gata5 in the development of various endodermal organs beyond the gut tube.
  • To elucidate the molecular mechanisms and genetic interactions of gata5 in early endoderm formation.

Main Methods:

  • Analysis of gata5 mutant zebrafish.
  • Gene expression analysis (sox17, axial/foxA2) in wild-type and mutant embryos.
  • Overexpression studies of gata5.
  • Genetic interaction analyses using mutants for gata5, bon, and cas.

Related Experiment Videos

Main Results:

  • gata5 mutants exhibit defects in liver, pancreas, thyroid, and thymus development.
  • gata5 is required for the generation of endodermal cells and maintenance of endodermal gene expression (sox17, axial/foxA2) during gastrulation.
  • Gata5 functions in conjunction with Bon and Cas, downstream of Nodal signaling, in endoderm formation.

Conclusions:

  • Gata5 plays multiple essential roles in zebrafish endoderm development, including cell proliferation and gene expression regulation.
  • Gata5 acts in a conserved pathway with Bon and Cas to control endoderm formation.
  • These findings position Gata5 as a key regulator within the broader network of endoderm development.