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Crystallization of the catalytic domain of murine terminal deoxynucleotidyl transferase
N Sukumar1, J B Boulé, N Expert-Bezançon
1Unité de Biochimie Structurale, Institut Pasteur, 25 Rue du Dr Roux, 75015 Paris, France.
Abstract:
The catalytic domain of murine terminal deoxynucleotidyl transferase (TdT) has been crystallized in the space group P2(1)2(1)2(1), with unit-cell parameters a = 47.1, b = 86.2, c = 111.7 A. The crystals diffract to a resolution of 2.4 A using synchrotron radiation and a full data set has been collected from the native crystals. The enzyme was shown to be active in the crystalline state.
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