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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
Published on: April 18, 2016
Low expression level but potent antigen presenting function of CD1d on monocyte lineage cells
F M Spada1, F Borriello, M Sugita
1Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
European Journal of Immunology
|November 28, 2000
Summary
Monocyte lineage cells are potent stimulators of CD1d-restricted immune responses, despite low surface expression. Their endocytic system is crucial for efficient antigen loading onto CD1d, highlighting unique CD1d regulation.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD1d is a crucial antigen-presenting molecule for Natural Killer T (NK T) cell selection and activation.
- NK T cells are a conserved T cell subset integral to immune responses.
Purpose of the Study:
- To investigate the expression, regulation, and function of human CD1d in myeloid-derived antigen-presenting cells (APCs).
- To understand the role of monocyte lineage cells in CD1d-restricted immune responses.
Main Methods:
- Analysis of CD1d expression and regulation in monocytes, monocyte-derived dendritic cells, and macrophages.
- Functional assays using NK T cell clones and the synthetic glycolipid antigen alpha-galactosyl ceramide.
- Microscopic localization studies of CD1d within monocyte lineage cells.
Main Results:
- CD1d is expressed as a mature glycoprotein by myeloid APCs, with constitutive expression not significantly upregulated by common cytokines.
- Despite low surface levels, myeloid cells are highly effective APCs for NK T cell responses to alpha-galactosyl ceramide.
- CD1d predominantly localizes to the endocytic system in monocyte lineage cells, facilitating efficient antigen loading.
Conclusions:
- Monocyte lineage cells are significant stimulators of CD1d-restricted immune responses.
- The unique regulation and endocytic localization of CD1d in these cells are critical for potent antigen presentation.
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