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Comparison of different protocols of MRC5 cell line permeabilization for detection of CMV IE antigens by flow
1Department of Virology, National Institute of Hygiene, Warsaw, Chocimska 24, Poland.
Abstract:
The usefulness of four different protocols of permeabilizing human fibroblast cells for detection of CMV immediate-early antigens by flow cytometry was investigated. Permeabilizing protocols employed methanol, methanol/acetone, paraformaldehyde/Triton and FACSPermeabilizing Solution (Becton Dickinson). The best parameters (separation between positive stained CMV infected cells and negative control and detection of infected cells) were achieved for paraformaldehyde/Triton and FACSPermeabilizing Solution (Becton Dickinson).
