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Expression of complement regulatory proteins in the developing human kidney.

D J Bridgewater1, A Mok, D G Matsell

  • 1Child Health Research Institute, Department of Anatomy and Cell Biology, University of Western Ontario, London, Canada.

Pediatric Nephrology (Berlin, Germany)
|November 30, 2000
PubMed
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Complement regulators CD35 and CD59 play roles in human kidney development. Their specific expression patterns in fetal and adult kidneys suggest involvement in renal cell differentiation and maturation.

Area of Science:

  • Nephrology
  • Immunology
  • Developmental Biology

Background:

  • Complement regulators CD35 and CD59 are key in host defense.
  • Emerging evidence suggests roles in human fetal kidney development and maturation.

Purpose of the Study:

  • To investigate the potential roles of CD35 and CD59 in kidney development.
  • To localize CD35 and CD59 protein and mRNA in developing and adult human kidneys.

Main Methods:

  • Immunohistochemistry and in situ hybridization were used to analyze adult and fetal kidney tissues.
  • Tissue ontogeny was examined to track developmental expression patterns.

Main Results:

  • CD35 protein and mRNA were found in glomerular podocytes of fetal and adult kidneys after vascularization.

Related Experiment Videos

  • CD59 protein and mRNA were detected early in gestation, primarily in fetal ureteric duct epithelium and adult collecting ducts.
  • CD59 showed a translocation from basolateral to apical surfaces between fetal and adult kidneys.

    • Conclusions:

    • The distinct spatial and temporal expression of CD35 and CD59 suggests their involvement in renal cell differentiation.
    • These findings highlight a novel role for complement regulatory proteins in kidney maturation.